Figure 5. ApoD is localized to atherosclerotic plaques ofapoE?deficient mice. Proximal aorta sections of male apoE knockout mice were subjected to oil red O staining (A), and to immunohistochemistry using control rabbit IgG against bacterial β-galactosidase (B) and rabbit anti-apoD antibody (C). The secondary antibody used in immunostaining is the donkey anti-rabbit IgG conjugated with Cy3. ApoD was stained red in theatherosclerotic plaque (C), as indicated by arrow. Elastic fibers ofvessels were auto-fluorescent blue, as indicated by arrowhead. Bar = 50 μm.