Research Paper Volume 1, Issue 1 pp 68—80
Fat accumulation in Caenorhabditis elegans triggered by the electrophilic lipid peroxidation product 4-Hydroxynonenal (4-HNE)
Figure 5. Effects of direct treatment with 4‑HNE on lipid accumulation in C. elegans. Synchronized L4 worms were kept in liquid culture for 3 days in medium containing the indicated concentration of 4‑HNE. The medium was replaced every 12 hr with fresh medium containing 4‑HNE. (A) fluorescence of worms treated with the indicated concentration of 4‑HNE but not stained (upper row of images), and animals exposed to 4‑HNE and stained with Nile red (lower row of images). (B) brightfield images of worms treated with the indicated concentration of 4‑HNE and directly photographed (upper row of images), or photographed after staining with Sudan black (lower row of images). (C): for each 4‑HNE concentration, lipid content was measured in three independently treated worm populations by quantitation of Nile red fluorescence (upper plot) or Sudan black staining (lower plot).