Research Paper Volume 1, Issue 1 pp 89—108
Alterations in gene expression and sensitivity to genotoxic stress following HdmX or Hdm2 knockdown in human tumor cells harboring wild-type p53
Figure 2. Loss of HdmX and/or Hdm2 inhibits MCF7 colony formation. (A) Following siRNA transfections, MCF7 cells were seeded at 500 cells/well in 6-well plates. The cells were allowed to grow for ten days then the colonies were stained with crystal violet. Significantly fewer colonies were present following knockdown of HdmX and/or Hdm2. The cells transfected with sip53 or a non-targeting control (siCon) showed minimal effects on colony formation relative to non-transfected control (Con/Control). (B) The percent cell growth relative to untransfected control was determined by extracting the stain in 10% acetic acid and quantifying the stain by reading absorbance at 590 nm.