Research Paper Volume 1, Issue 1 pp 89—108
Alterations in gene expression and sensitivity to genotoxic stress following HdmX or Hdm2 knockdown in human tumor cells harboring wild-type p53
Figure 7. Repression of E2F1-regulated genes by Hdm2 or HdmX knockdown is blocked by concurrent knockdown of p21. MCF7 cells were transfected with the indicated siRNA combinations. Twenty-four hours later, RNA was isolated and subjected to RT-qPCR to quantify expression of CCNA2, p21 and E2F1 after normalization to GAPDH. Expression levels (Y-axis) were relative to siCon and reported as RQ values. Error bars represent the 95% confidence interval of the relative expression.