Research Paper Volume 1, Issue 1 pp 109—121

Figure 3. SIRT6 is required for global deacetylation of H3K9 in response to DNA damage. (a) Western analysis of SIRT6 expression levels in the indicated cell lines stably expressing two different SIRT6 shRNAs (S6KD1 and S6KD2) or empty vector control (pSR). (b) SIRT6 knock-down leads to hypersensitivity to γ-irradiation (IR). Gy, Gray. (c) Western analysis of H3K9Ac levels in S6KD2 (KD) or control (Co) HT1080 cells in response to DNA DSB agents neocarzinostatin (NCS) or bleomycin (Bleo) treatment (1hr). (d) Western analysis of H3K9Ac levels in 293T cells over-expressing SIRT6 or empty vector control (pcDNA), following exposure to NCS (1hr). In (c) and (d), total H3 or H3K14Ac levels are shown as controls.