Research Paper Volume 1, Issue 1 pp 109—121
SIRT6 stabilizes DNA-dependent Protein Kinase at chromatin for DNA double-strand break repair
Figure 7. SIRT6 is not required for DNA double-strand break rejoining in a cell-free system. (a) Western analysis showing immunodepletion of SIRT6 from HeLa cell nuclear extracts (NE) with anti-SIRT6 antibodies. Mock-depleted control nuclear extracts were generated using the protein A/G-sepharose beads alone. (b) Ethidium bromide stain (inverted image) of DNA products of cell-free DSB rejoining assay. Reactions contained linearized pUC19 plasmid DNA fragments and SIRT6- or mock- depleted nuclear extracts. No difference in ligated species is observed between SIRT6-depleted and mock-depleted reactions.