Research Paper Volume 1, Issue 1 pp 131—145

Figure 4. Reduced TOR signaling does not rescue chronological life span in the short-lived GS129 strain with imbalanced mitochondrial translation. (A) Results of mitochondrial translation assays are shown as described in Figure 1. The strains analyzed are GS122 (wt with regard to RPO41) and GS129 (containing the rpo41-R129D point mutation) in which the TOR1 gene was (tor1Δ) or was not (wt) disrupted (see Materials and Methods). (B) Chronological life span curves of the same strains in A. are shown. Three independent colo-nies of each strain were analyzed and the mean % viability +/- one standard deviation is plotted according to the key in the lower left corner.