Research Paper Volume 1, Issue 12 pp 1008—1016

Figure 5. Correlation between S6 phosphorylation, hypertrophy and loss of proliferative potential in senescent cells. HT-p21 cells were plated in 6 well plates and treated with IPTG plus the increasing concentrations of rapamycin (from 0.16 to 500 nM). At concentration 0, cells were treated with IPTG alone. (A) Cellular hypertrophy: protein and GFP. After 3 days, soluble protein and GFP were measured per well. [Note: in non-proliferating cells, protein/well is a measure of protein/cells]. Results are shown as percent of IPTG alone (0) without rapamycin. (B) After 3 days, cells were lysed and immunobloted for p-S6, S6 and p21. (C) PC: preservation of proliferative competence. After 3 days, cells were washed to remove IPTG and RAPA. Cells were incubated for additional 5 days in the fresh medium and then were counted. Results are shown as percent of IPTG alone (0) without rapamycin.