Research Paper Volume 1, Issue 12 pp 1008—1016
Quantifying pharmacologic suppression of cellular senescence: prevention of cellular hypertrophy versus preservation of proliferative potential
Figure S1. Induction of p21 by IPTG. HT-p21 cells were plated in 6 well plates and treated with IPTG with or without rapamycin as indicated. The next day, cells were lysed and immunoblot for p-S6, S6 and p21 was performed as described in Methods. IPTG dramatically induced p21, without affecting S6 phosphorylation, whereas rapamycin inhibited S6 phosphorylation, without affecting p21 induction.