Figure S2. Loss of hypertrophy following release. HT-p21 cells were treated with IPTG plus 500 nM rapamycin for 3 days. Then the cells were washed and the cells were incubated in the fresh medium without drugs. At indicated days, soluble protein, GFP and cell numbers were measured per well. Protein (pr) per cell and GFP per cell were calculated and plotted in arbitrary units.