Research Paper Volume 2, Issue 12 pp 990—998

Statin prevents chondrocyte aging and degeneration of articular cartilage in osteoarthritis (OA)


Figure 2. Effects of statin on the production of matrix degrading enzymes and proteo-glycan in chondrocytes. Chondrocytes were incubated with catabolic factor, IL-1β (10 ng/ml) in the presence or absence of statin (1.0, 10.0 μM). After 48 hour-incubation, the concentrations of matrix degrading enzymes and proteoglycan were analyzed by ELISA (A: MMP1, B: MMP-3, C: MMP-13, D: proteoglycan). IL-1β stimulated the production of MMP-1, -3, and -13 by chondrocytes in control cultures (* p< 0.05 compared to each control). Statin at 10.0 μM significantly decreased the IL-1β- induced excess production of MMP-1 and -13, but not MMP-3, from chondrocytes (** p< 0.01, compared to IL-1β-stimulated group). IL-1β showed a tendency to decrease the production of proteoglycan by chondrocytes. Statin at 10.0 μM cancelled the IL-1βinduced decreases of proteoglycan production from chondrocytes in both culture conditions (** p< 0.0, compared to IL-1β-stimulated group). Data from four independent experiments were analyzed.