Research Paper Volume 2, Issue 11 pp 785—790

Figure 2. CENP-A expression in mouse beta-cells as a function of age. Representative low power (a-c) and corresponding high power (d-f) images from mouse pancreases of different ages immunostained for CENP-A (green) and insulin (red). Quantitative analysis of CENP-A and insulin double positive cells as a function of age (1 month old mice, N=4; 7 month old mice, N=4; 16 month old mice, N=3). Data are mean ± SEM (g). The number of foci of CENP-A staining per beta-cells was quantitated. More than 20 islets were counted from each mouse. Low power (h-j) and corresponding high power (k-m) images from mouse pancreases immunostained for CENP-A (green), insulin (blue) and Ki67 (red). Arrowheads (high power views) and corresponding arrows (low power views) indicate dividing beta-cells (positive for insulin and Ki67) that also exhibit a high level of CENP-A (green). Blue nuclear counterstain is DAPI. Scale bars, a-c=50uM, d-f=10uM, h-m=50uM.