Research Paper Volume 2, Issue 11 pp 823—842

Figure 6. HIF-1α silencing normalizes glycolytic metabolism but not mitochondrial activity of Stat3^C/C MEFs. Empty bars or filled bars, Stat3^WT/WT or Stat3^C/C MEFs respectively, either silenced or not for HIF-1α (sh-HIF-1α), represent mean values ± s.e.m. of three independent experiments. *, p ≤ 0,001. (A) Taqman RT-PCR quantification of the indicated mRNAs. (B-D) Lactate production, glucose intake and sensitivity to glucose deprivation were measured as described in the legend to Fig. 3. (E) Mitochondrial Ca²⁺ homeostasis, measure as described in the legend to Figure 4.