Figure 4. Activation of mitochondrial metabolism sustains activation of the insulin signaling cascade following doxorubicin-induced cardiomyopathy. Three animals were analyzed by western blotting for each group. Lanes 1-3: WT untreated; lanes 4-6: FX untreated; lanes 7-9: WT doxorubicin-treated; lanes 10-12: FX doxorubicin-treated. Membranes were probed with the indicated antibody, then stripped and re-probed with α-tubulin as loading control. Approximate protein band size to the left as indicated by arrows.