Research Paper Volume 2, Issue 12 pp 908—913

Figure 3. Age-dependent malignancy of transformed hematopoietic progenitor cells in vivo. (A) Kaplan-Meier survival analysis. Animals where BCR-ABL expression was induced at 4-month of age survived significantly longer than animals where BCR-ABL expression was induced at 12- or 20-month of age (median survival of 88 days versus 50.5 and 33 days, respectively; log rank P < 0.0001 and 0.0001, respectively). (B) CombitTA-p190 mice were evaluated for disease progression by flow citometry. Cells from peripheral blood of CombitTA-p190 and control mice were analyzed by flow cytometry with combination of the specific myeloid (Mac1) and B-cell lymphoid (B220) markers. A representative flow cytometry analysis is shown Characteristic blast cells in BCR-ABL B-cell leukaemia co-expressed B-cell and myeloid markers indicated by the presence of Mac⁺B220⁺ cells. (C) Variability on the percentage of blast cells in the preripheral blood as a function of donor age.