Research Paper Volume 2, Issue 12 pp 924—935
DNA damaging agents and p53 do not cause senescence in quiescent cells, while consecutive re-activation of mTOR is associated with conversion to senescence
Figure 8. Serum stimulation converts Dox-locked quiescence into senescence in WI-38t cells. WI38t cells were treated with 100 ng/ml doxorubicin (low-Dox) or left untreated in serum-free medium (no serum) for 3 days, and then 10% serum was added. After 3 days of serum stimulation cells were stained for beta-gal and microphotographed. Bar - 50 micron.