Research Paper Volume 3, Issue 8 pp 794—802
Altered behavioral and metabolic circadian rhythms in mice with disrupted NAD+ oscillation
Figure 3. Differential liver gene expression in CD38-null mice. Mice entrained in 12 hr Light - 12 hr Dark cycles were sacrificed at indicated times and their liver was dissected out.(A) RNA was prepared at indicated times, reverse transcribed, and real-time PCR was performed using primers for Dbp, Per2, Nampt and 18S rRNA. Data is represented as relative levels of indicated gene normalized to 18S rRNA.(B) Same as in (A), except real-time PCR was performed using primers for Asns, Igfbp1, Moxd1 and c -Myc. Data is represented as relative levels of indicated gene normalized to 18S rRNA. *, p<0.05 compared to the corresponding wild type, n= 3 each time point.