Research Paper Volume 3, Issue 11 pp 1078—1091

Yeast-like chronological senescence in mammalian cells: phenomenon, mechanism and pharmacological suppression


Figure 4. Effects of NAC and CGK733 on CS and mTOR. (A) HT-p21-9 cells were plated at 40000 per well in 96 well plates with indicated agents: 500 nM rapamycin (R), 5-20 mM NAC and incubated for 6 days. On Day 6 replicative viability of cells was measured by colony formation (8 days) as described in legend for Figure 1. (B) HT-p21-9 cells were plated at high density with indicated agents or left untreated (C) and allowed to attach for 6 hrs. Then, one set was lysed (Day 0, 6 hr) and second set was lysed after 24 hrs treatment (Day 1). Immunoblot was performed using indicated antibodies.