Research Paper Volume 3, Issue 11 pp 1078—1091

Yeast-like chronological senescence in mammalian cells: phenomenon, mechanism and pharmacological suppression


Figure 9. Effects of initial density and rapamycin on cell proliferation, survival and replicative viability. Experiment was performed as described in the legend for Figure 8 (Upper panel). Cell survival. Alive (attached) and dead (floating) cells were counted in all wells on Day 4. (Lower panel). Replicative viability. In all samples, attached cells were replated in the fresh medium in low density and viability was measured by trypsinizing colonies and counting cells.