Research Paper Volume 3, Issue 12 pp 1169—1177
Peripheral oxytocin treatment ameliorates obesity by reducing food intake and visceral fat mass
Figure 1. Ip injection of Oxt suppresses food intake and activates neurons in the hypothalamus and brain stem in standard diet fed mice. (A) Food intake after ip injection of Oxt. White, gray and black bars indicate saline (n = 5), 200 μg/kg dose of Oxt (n = 5) and 400 μg/kg dose of Oxt (n = 5), respectively. (B-E) C-Fos expression in the PVN (B), ARC (C), LC (D), and the region including NTS, DMX and AP (E) after saline (each left panel) or 400 μg/kg dose of Oxt (each right panel) injection. cc: central canal. (F) Number of c-Fos immunoreactive neurons in the feeding-related areas of the hypothalamus and brain stem after injection of 400 μg/kg dose of Oxt (filled bars) or saline (open bars). n = 5 for Oxt and for control. *p < 0.05, **p < 0.01.