Research Paper Volume 3, Issue 12 pp 1213—1223

Figure 2. Effects of the MK2 inhibitor on the expression of GSTT1 in KGN cells stimulated with H₂O₂ Cells were treated with H₂O₂ at 200 μM with or without SB203580 at 10 μM or CMPD1 at 330 nM for 24 h and subjected to immunofluorescence analysis (A). The primary antibody against GSTT1 was probed with anti-rabbit IgG-Alexa488 (Green). Cells were counterstained with Hoechst 33342 at 10 μM (Blue). Magnification: ×200. A bar graph represent the mean fluorescence intensity per cell ± SEM (B, C). One-way ANOVA: (B) P < 0.05; (C) P < 0.01.