Research Paper Volume 3, Issue 12 pp 1213—1223
GSTT1 is upregulated by oxidative stress through p38-MK2 signaling pathway in human granulosa cells: possible association with mitochondrial activity
Figure 4. Depletion of GSTT1 prevents the cytoplasmic activation of p38. Cells stimulated with or without H2O2 were subjected to fractionation of cytosolic and nuclear proteins. The activity of p38 in each fraction was then analyzed by immunoblotting (A: cytoplasm, B: nuclei). Fifteen micrograms of total protein were used for each lane. Bar graphs represent the mean band intensity ± SEM. (A) Student's t–test: P < 0.05.