dSir2 deficiency in the fatbody, but not muscles, affects systemic insulin signaling, fat mobilization and starvation survival in flies

Kushal Kr. Banerjee; Champakali Ayyub; Samudra Sengupta; Ullas Kolthur-Seetharam

doi:doi:http://dx.doi.org/10.18632/aging.100435

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Research Paper Volume 4, Issue 3 pp 206—223

dSir2 deficiency in the fatbody, but not muscles, affects systemic insulin signaling, fat mobilization and starvation survival in flies

Figure 4. Fatbody dSir2, but not muscle specific dSir2 regulates starvation survival and regulates fat mobilization.

(A and B) Starvation survival in (A) fatbody dSir2 knockdown flies (p < 0.001) and (B) muscle dSir2 knockdown flies (non-significant, ns)(n = 60). 200 μM RU486 was used to knockdown dSir2 expression pSw-S₁106-gal4>dSir2^RNAi and pSw-MHC-gal4> dSir2^RNAi flies. Log Rank was used to plot survival curves and Mantel-Cox test was used for statistical analysis. (C-D) Total body triglyceride levels in fed and starved conditions in (C) whole bodydSir2^RNAiflies, with respective controls (n = 36) and (D) fatbody dSir2^RNAi (n = 36). Relative expression of fat metabolism genes (E) lipase-3 (lip3), (F) brummer (brmm), (G) medium chain acyl CoA dehydrogenase (mcad), (H) fatty acid synthase (fas) in fatbody dSir2 knockdown flies under fed and starved conditions (n = 24). 200 μM RU486 was used to knockdown dSir2 expression pSw-S₁106-gal4>dSir2^RNAi and pSw-MHC-gal4> dSir2^RNAi flies. ANOVA was used to analyze statistical significance of the data (*, p < 0.05; **, p < 0.01; ***, p < 0.001 or mentioned otherwise).