Research Paper Volume 4, Issue 4 pp 290—304

ATM-dependent phosphorylation of SNEVhPrp19/hPso4 is involved in extending cellular life span and suppression of apoptosis

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Figure 3. Upon oxidative stress, SNEV is phosphorylated and localizes mainly to the nucleus.

(A) Hela cells were seeded on coverslipsand treated with 100 μMH2O2 for 1 hour. Cells were stained with anti-pSNEV(S149) and DAPI as described in the Materials and Methods section and subjected to fluorescence microscopy. (B) HeLa cells were treated with 25 μg/ml MMC for 24 hours prior to indirect immunofluorescence staining with anti-pSNEV(S149) antibody.