Research Paper Volume 4, Issue 4 pp 290—304
ATM-dependent phosphorylation of SNEVhPrp19/hPso4 is involved in extending cellular life span and suppression of apoptosis
Figure 3. Upon oxidative stress, SNEV is phosphorylated and localizes mainly to the nucleus.
(A) Hela cells were seeded on coverslipsand treated with 100 μMH2O2 for 1 hour. Cells were stained with anti-pSNEV(S149) and DAPI as described in the Materials and Methods section and subjected to fluorescence microscopy. (B) HeLa cells were treated with 25 μg/ml MMC for 24 hours prior to indirect immunofluorescence staining with anti-pSNEV(S149) antibody.