Research Paper Volume 4, Issue 9 pp 620—635

Figure 2. Aging causes loss of mitochondrial biogenesis signaling.

qPCR analyses of mitobiogenesis gene expression levels in cDNA's from young (5 month, n=6) and old (21 month, n=9) brains (A), hearts (B) and skeletal muscle (C), expressed as % mean young mouse levels. In brain, expression of all genes in young brains were different from that in old brains at p<0.0001 (unpaired t-test). In heart, expression of TFBM2 (p=0.0325) and PGC-1α (p<0.0001) were significantly different between young and old animals (unpaired t-test). Expression of TFAM and NRF2 were not significantly different. In muscle, expression of TFAM (p=0.012) and TFBM2 (p=0.036) in young mice were significantly different from old mice; expression of PGC1α and NRF2 were not significantly different between young and old mice (unpaired t-test).