Research Paper Volume 4, Issue 10 pp 664—673

Figure 4. CBS overexpression delays premature senescence in human endothelial cells.

(A) Left panel: Cumulative population doublings of CBS overexpressing (OE) and control HUVEC were calculated, beginning 11 days after infection. Growth curves represent three independent experiments in each case. Right panel: CBS Western blot after lentiviral infection with the CBS overexpression and control construct. (B) Left panel: Bar diagram displaying relative percentage of SA-ß-gal positive cells 46 days after lentiviral infection with the CBS overexpression and control construct. Data are represented as mean ± SE (n=3). ***: p<0.001 Right panel: Representative micrographs of cells stained for SA-ß-gal activity.