Research Paper Volume 4, Issue 10 pp 695—708

Figure 1. The levels of RBP NF90 are lower in senescent cells.

(A) Western blot analysis of the expression levels of RBPs HuR, AUF1, NCL and NF90 in whole-cell lysates prepared from human diploid WI-38 fibroblasts ['young', proliferating (P, pdl 20) or senescent (S, pdl 54)] and from human IDH4 fibroblasts [P (+Dex) or S (-Dex for 9 days)]; the levels of GAPDH were assessed as a loading control. (B) Western blot analysis of the senescence-associated cdk inhibitor p21 in cells from the populations described in panel A. (C,D) In the WI-38 (C) and IDH4 (D) cell populations described in panel A, total RNA was prepared and the levels of mRNAs encoding the SASP factors shown were measured by RT-qPCR. Data are the means and standard deviation (+SD) from three independent experiments.