Research Paper Volume 4, Issue 10 pp 695—708

Figure 2. NF90-associated SASP mRNAs.

(A) Lysates were prepared from proliferating WI-38 (P) and IDH4 (+Dex) cells and subjected to immunoprecipitation (IP) using either anti-NF90 or IgG antibodies, whereupon Western blot (WB) analysis was used to detect NF90 levels in the IP samples. (B,C) The levels of SASP mRNAs in the ribonucleoprotein (RNP) complexes present in the IgG IP and NF90 IP samples from panel A were measured by RT-qPCR analysis. The levels of enrichment of SASP mRNAs in NF90 IP relative to IgG IP were calculated. Enrichments >2-fold are shown for WI-38 cells (B) and enrichments >1.5-fold are shown for IDH4 cells (C). In (B,C) data are the means +SD from three independent experiments.