Research Paper Volume 4, Issue 10 pp 695—708

Figure 3. Silencing NF90 increases fibroblast sense-cence, decreases proliferation.

(A) Forty-eight h after transfecting WI-38 cells (pdl 30) and IDH4 cells (+Dex) with either NF90 siRNA or Ctrl siRNA, whole-cell lysates were prepared and the levels of senescence markers p21, p53, and p16 were studied by Western blot analysis. (B) WI-38 and IDH4 cells were transfected as explained in panel A; two weeks later cells were counted and plotted. Data represent the means +SD from three independent experiments.