Research Paper Volume 4, Issue 10 pp 695—708

NF90 coordinately represses the senescence-associated secretory phenotype

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Figure 4. Influence of NF90 on the steady-state expression of SASP mRNAs.

(A) Forty-eight h after transfecting WI-38 cells (pdl 30) with Ctrl siRNA or NF90 siRNA, total RNA was prepared and the levels of SASP mRNAs were quantified by RT-qPCR analysis (normalized to 18S rRNA levels). (B) Forty-eight h after transfecting IDH4 cells (+Dex) with Ctrl siRNA or NF90 siRNA, total RNA was prepared and the levels of SASP mRNAs were quantified by RT-qPCR analysis (normalized to 18S rRNA levels). Data represent the means +SD from three independent experiments.