Research Paper Volume 4, Issue 10 pp 709—714
Rapamycin extends lifespan and delays tumorigenesis in heterozygous p53+/− mice
Figure 2. Administration of rapamycin in drinking water inhibits the mTOR pathway in p53+/− male mice.
(A) Western blot analysis of whole cell lysates of 6 organs of rapamycin-treated and control mice probed with antibodies specific to S6 and phospho-S6 (Ser240/244). Mice received rapamycin in drinking water for 2 days. (B) Immunohistochemistry. pS6 in the heart and the liver. Mice received rapamycin in drinking water for 2 days.