Research Paper Volume 4, Issue 11 pp 768—789
Gene expression changes in response to aging compared to heat stress, oxidative stress and ionizing radiation in Drosophila melanogaster
Figure 2. GstD-GFP transgenic reporters recapitulate GstD1 gene induction during aging.
(A) Expression of the transgenic reporter constructs GstD-GFP and GstD-deltaARE-GFP was visualized in 6 day old (Young) and 30 day old (Old) male and female flies, as indicated, using the fluorescence stereomicroscope. The GFP image and an overlay of the GFP image and the visible light image are presented, as indicated. (B) Quantification of the expression of the GstD-GFP and GstD-deltaARE-GFP reporters in male (M) and female (F) flies, both young (Y) and old (O), as indicated. The data for the Gst-deltaARE-GFP reporter is specified by prefix (delta). Values for old flies were compared to young using unpaired, two-sided t-tests, and statistically significant differences (p < 0.05) are indicated with asterisk.