Figure 2. Aging inhibited LC maturation.
Epidermal cells were cultured in RPMI for 48h, and then stained with anti-Langerin, MHCII, CD80, CD86 antibodies, and analyzed by flow cytometry. The expression of MHCII and co-stimulatory molecules was determined by the ratio (A) and mean fluorescence intensity (MFI) (B). Data are shown as mean ± SEM of 6-10 mice each group.