Research Paper Volume 4, Issue 11 pp 742—754

Aging affects epidermal Langerhans cell development and function and alters their miRNA gene expression profile

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Figure 2. Aging inhibited LC maturation.

Epidermal cells were cultured in RPMI for 48h, and then stained with anti-Langerin, MHCII, CD80, CD86 antibodies, and analyzed by flow cytometry. The expression of MHCII and co-stimulatory molecules was determined by the ratio (A) and mean fluorescence intensity (MFI) (B). Data are shown as mean ± SEM of 6-10 mice each group.