Research Paper Volume 4, Issue 11 pp 742—754

Figure 4. Aged mice exhibit defects in LC-mediated T cell proliferation.

LCs derived from aged and young mice were pulsed for 20 hr at 37°C with OVA protein (0.5mg/ml) then washed to remove excess OVA. LCs were first purified using AutoMACS with anti-MHCII-PE and anti-PE microbeads followed by a cell sorter. OT-I specific CD8 T cells purified from OT-I mice or OT-II specific CD4 T cells purified from OT-II mice were cultured with LCs for 72h. 1μCi of [³H] thymidine/well was added during the last16-18h. Cells were then harvested and radioactivity counted. Data are representative of two experiments.