Research Paper Volume 4, Issue 11 pp 742—754

Aging affects epidermal Langerhans cell development and function and alters their miRNA gene expression profile

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Figure 5. Aging regulates miRNA gene expression profiles in epidermal LCs.

(A) LCs were isolated using AutoMACS with anti-MHCII-PE and anti-PE microbeadsfollowed by a cell sorter. The purity was confirmed to be ~98%.(B) Expression profiles of miRNAs in young and aged LCs from two individual experiments. (C) miRNA expression in LCs was significantly up- and downregulated in aged mice compared to that in young mice. (D) Changed miRNAs in aging were confirmed by single TaqMan RT-PCR.