Research Paper Volume 4, Issue 11 pp 803—822

Figure 4. Cardiomyocytes derived from LMNA^R225X/WT, LMNA^{Frameshift/WT} and LMNA^WT/WT iPSCs.

(A, B & C) Beating embryoid bodies derived from LMNA^R225X/WT, LMNA^{Frameshift/WT} and LMNA^WT/WT iPSCs;(D, E & F) After micro-surgical dissection, spontaneously beating cell clusters were plated onto glass coverslips. Videos of these beating embryoid bodies and clusters were available in supplemental materials. (G, H & I) Immunofluorescence co-staining showing the expression of cardiac specific marker, α-actinin and lamin A/C in these cells, (J, K & L) Electronic microscopy of nucleus of cardiomyocytes derived from LMNA^R225X/WT, LMNA^{Frameshift/WT} and LMNA^WT/WT iPSC line. The black arrows indicate the loss of nuclear envelope in the LMNA^R225X/WT iPSC-derived cardiomyocytes.