Research Paper Volume 5, Issue 1 pp 51—66
Chronic oxidative stress upregulates Drusen-related protein expression in adult human RPE stem cell-derived RPE cells: A novel culture model for dry AMD
Figure 2. Oxidative stress reduces hRPE cell viability and disrupts transepithelial resistance.
(A) hRPE (n=4) release a greater percentage of total intracellular LDH in response to TBHP treatment than ARPE-19 cells (n=4) in the first two days, but both cell types responded similarly over the following days of TBHP treatment (Student's t-test, **, p<0.01). (B) The maximum achievable TER of the hRPE was significantly greater than that of the ARPE-19 under these culture conditions (Student's t-test, **, p<0.01). The TER decreased after multiple TBHP treatments (Student's t-test, *, p<0.05, **, p<0.01; Error bars represent SEM).