Research Paper Volume 4, Issue 12 pp 952—965

Potential anti-aging agents suppress the level of constitutive mTOR- and DNA damage- signaling


Figure 6. Effect of treatment of mitogenically stimulated human lymphocytes with RAP, BRB, Vit. D3, RSV or ASA for 4 h on the level of constitutive expression of RP-S6P.

Peripheral blood lymphocytes were mitogenically stimulated with phytohemagglutinin (PHA) for 72 h, the cells were then treated with the respective drugs at concentrations as shown in Figs. 1 and 2 for 4 h, RP-S6 wasdetected immunocytochemically and cellular fluorescence measured by flow cytometry. The bar graphs present the mean values (+SD) of RP-S6P immunofluorescence for G1, S and G2M cell subpopulations identified by differences in DNA content (intensity of DAPI fluorescence).