Research Paper Volume 4, Issue 12 pp 952—965

Potential anti-aging agents suppress the level of constitutive mTOR- and DNA damage- signaling


Figure 8. Effects of the studied gero-preventive agents on the intercellular level of ROS.

TK6 cells, untreated (Ctrl) or treated for 24 h with the investigated agents, were exposed for 30 min to H2DCF-DA and their fluorescence intensity was measured by flow cytometry. The cell-permeant non-fluorescent H2DCF-DA upon cleavage of the acetate moiety by intercellular esterases and oxidation by ROS is converted to strongly fluorescent DCF and thus reports the ROS abundance. Left panel shows the frequency histograms of the untreated (Ctrl) as well MF and RAP-treated cells (note exponential scale of the DCF fluorescence). Right panel presents the mean values (+SD) of DCF fluorescence of the untreated (Ctrl) and treated cells.