Research Paper Volume 4, Issue 12 pp 952—965

Potential anti-aging agents suppress the level of constitutive mTOR- and DNA damage- signaling


Figure 9. Effect of the studied gero-preventive agents on the mitochondrial transmembrane potential (ΔΨm).

TK6 cells, untreated (Ctrl) or treated for 24 h with the investigated agents were exposed for 30 min to the mitochondrial probe rhodamine 123 (Rh-123) and their fluorescence intensity was measured by flow cytometry. Left panel shows the frequency histograms of the untreated (Ctrl) as well MF and RAP-treated cells (note exponential scale of the DCF fluorescence). Right panel presents the mean values (+SD) of Rh-123 fluorescence of the investigated cells.