Research Paper Volume 4, Issue 12 pp 966—977

Figure 1. LAD ligation in newborn mice results in massive cardiac damage.

(A) Incisions into the left chest wall were used to open the chest cavity and irreversibly ligate the left anterior descending artery (LAD) in newborn mice. Representative images are shown. In Sham-operated mice the chest cavity was opened, without LAD ligation. (B-D) Massive cardiac damage 24 hours after the initial LAD ligation as demonstrated by H&E staining (B), immune-detection of cleaved Caspase 3 (cC3) (B), loss of TroponinT expression (negative = infarction area, asterisk) (B), and echocardiography (D). Arrows in B indicate infarction area. Data from sham operated newborn mice are shown as controls. In (B) and (B) images are representative of 5 mice analyzed. Data in (D) are shown as mean values +/− SEM (n=6 per group). Arrows in (D) indicate LVESD and LVEDD. Probability values were determined using a Mann-Whitney U Test.