Research Paper Volume 5, Issue 1 pp 37—50
Specific lipofuscin staining as a novel biomarker to detect replicative and stress-induced senescence. A method applicable in cryo-preserved and archival tissues
Figure 6. Lipofuscin accumulates and co-localizes with Senescence-Associated beta-galactosidase (SA-β-gal) in senescent cells detected in cryo-preserved material from benign prostatic hyperplasia (BPH).
Frozen material from patients with BPH in enlarged prostates (prostate weight greater than 55gr) was thin-sectioned (5 μm). The sections were immediately double stained for SA-β-gal activity (turquoise color) and Nuclear Fast Red (NFR) as counterstain (A) and double stained with SBB and NFR (B). Areas with characteristic BPH pathology showed SA-β-gal activity and lipofuscin positivity (C). Normal prostate regions adjacent to BPH, were found negative for SA-β-gal activity and lipofuscin (D). Scale bars: A-C, 100 μm; D, 50 μm. Insets: Cells at higher magnification.