Research Paper Volume 5, Issue 2 pp 120—129

How to measure RNA expression in rare senescent cells expressing any specific protein such as p16Ink4a

class="figure-viewer-img"

Figure 1. Expression of p16 at the single cell level measured by immunostaining with a p16 antibody followed by immunohistochemical detection.

(A) Non-immortalized HDF (LF1) at early passage. (B) LF1 HDF passaged into senescence. (C) LF1 cells immortalized with telomerase (LF1/TERT) under conditions of exponential proliferation.