Research Paper Volume 5, Issue 3 pp 155—173

Figure 1.

Detection of PrP in brains from five cases with six extra octapeptide repeats using Western blotting with 3F4. (A) Brain samples were from five cases (1 through 5) and were not treated with PK. T1: PrP^Sc type 1 control; T2: PrP^Sc type 2 control. (B) Western blot of β-actin, which was used to monitor the amounts of samples from each case. (C) The samples were treated with PK prior to SDS-PAGE and immunoblotting. The gel mobility of the PK-resistant PrP from the cases 1, 2, and 4 was similar to that of PrP^Sc type 1 control migrating at ~21 kDa while the case 3 was similar to PrP^Sc type 2 migrating at ~19 kDa. No PK-resistant PrP was visible in the case 5. (D) An over exposed smaller blot from the left part of the blot shown in C. The PK-resistant PrP bands from case 5 became detectable, the gel mobility of which was similar to that of case 2 migrating at ~19 kDa.