Research Paper Volume 5, Issue 3 pp 155—173

Figure 7.

Two-dimensional Western blotting of PrP. (A) Comparison of untreated PrP from case 6 with fCJD^Ins+sPrPSc and non-CJD and sCJD. PrP from fCJD^Ins includes 2D spots I-IV and PrP from non-CJD mainly consists of 2D spots I and IV. (B) Comparison of PNGase F-treated PrP from fCJD^Ins and non-CJD. PrP from the two conditions comprises both full-length PrP (2D spots III) and N-terminally truncated PrP (2D spots VI); however, PrP from fCJD^Ins contains additional set of PrP spots (2D spots III’). The blots were probed with 3F4.