Research Paper Volume 5, Issue 4 pp 288—303
An inhibitory role of progerin in the gene induction network of adipocyte differentiation from iPS cells
Figure 3. HGPS adipocytes exhibit characteristic cellular phenotypes.
(A) Immunofluorescence images of normal and HGPS adipocytes with lamin A/C staining in red and progerin staining in green at the fourth week differentiation. Nuclei are counterstained in blue with Hoechst 33342. Scale bar: 50 μm (B) Upper panel: Representative images of blebbed nucleus stained with progerin antibody and Hoechst 33342. Lower panel: Histogram shows the percentage of blebbed nuclei. (C) Upper panel: Representative images of binucleated nuclei stained with progerin antibody and Hoechst 33342. Lower panel: Histogram shows the percentage of binucleated nuclei. (D) Senescence-associated (SA)-β-Gal stainings of normal and HGPS adipocytes at the fourth week differentiation shows premature cellular senescence. Scale bar: 100 μm. Cell lines: Normal (8470) and HGPS (164).