Research Paper Volume 5, Issue 4 pp 288—303
An inhibitory role of progerin in the gene induction network of adipocyte differentiation from iPS cells
Figure 4. Progerin suppresses the transcription activation of core adipogenic regulators during adipocyte differentiation.
(A) and (B) Quantitative RT-PCR analysis of early adipogenic genes C/EBPβ and C/EBPδ (A) and late adipogenic genes PPARγ2 and C/EBPα (B) in normal and HGPS iPSCs and adipocytes. n = 3. ** p < 0.01. (C) Western Blotting analysis with lamin A/C, PPARγ2, and actin antibodies in normal and HGPS iPSCs and adipocytes. Red filled triangle points to progerin. (D) Western Blotting analysis with C/EBPα and actin antibodies in normal and HGPS iPSCs and adipocytes. Cell lines: Normal (8470) and HGPS (164).