Research Paper Volume 5, Issue 4 pp 315—327

Figure 2. Fat body Sir2 does not play a major role in the DR response.

Transcriptional profiles of fat bodies from either w1118 females after a 7 d DR period or UAS-Sir2>S₁106-Gal4 females after 24 h of Sir2 overexpression were compared. The scaled Venn diagrams depict the actual relationship of the numbers of up-regulated (A) and down-regulated genes (B) of both samples and the number of overlapping genes. Only roughly 10 % of differentially expressed genes are overlapping between DR samples and Sir2 overexpression. Statistical analyses were performed with Fisher's exact test. Genes, which were up-or downregulated > 1.5 fold in at least 2 of 3 microarrays were included in the diagrams shown. Hierarchical clustering of DR and Sir2 Arrays reveals that transcriptional patterns elicited by DR and Sir2 overexpression in the fat body do not show substantial similarities. (D) mRNA levels of Sir2 in female w¹¹¹⁸ whole animals and in their fat bodies were unchanged after DR. Shown are mean values of 3 replicates + SEM.