Research Paper Volume 5, Issue 6 pp 460—473

Figure 2. QRT-PCR quantification of miRNA identified as down-regulated in the kidney of old WT and progeroid Ercc1^−/Δ mice compared to adultWT kidney.

QRT-PCR analysis was performed on kidneys of WT young (20 weeks), Ercc1^−/Δ (20 weeks), and WT old mice (30 months). (A) miR-449a. (B) miR-455*. (C) miR-128. All three miRNAs identified in the microarray were significantly downregulated in kidney tissue of Ercc1^−/Δ progeroid mice and old mice compared to WT young mice. No RT, no reverse transcriptase added. Three mouse kidneys are in each condition. The mean of three experimental replicates for each sample is graphed as relative to WT young samples, which were normalized to a value of -1. The standard deviation is plotted as error bars. P-values were calculated comparing samples to WT Young using Welch's t-tests and are indicated by * (p < .05), ** (p< .01), *** (p< .001) and # (p<.0001).