Research Paper Volume 5, Issue 7 pp 539—550
Rapamycin doses sufficient to extend lifespan do not compromise muscle mitochondrial content or endurance
Figure 2. Rapamycin has no major effects on mitochondrial protein levels.
Representative subunits of each electron transport complex were detected by Western blotting using a cocktail of monoclonal antibodies from MitoSciences. Because the complex IV subunit was not detected using the cocktail, a separate COXIV antibody was also used. The identities of the probed subunits are as follows: complex I - NADH dehydrogenase 1 beta subcomplex 8 (NDUFB8); complex II - succinate dehydrogenase subunit B (SDHB); complex III - ubiquinol-cytochrome c reductase core protein 2 (UQCRC2); complex V -ATP synthase subunit alpha (ATP5A). Proteins were measured in (A) soleus or (B) gastrocnemius (gastroc) muscle following 2 weeks of rapamycin treatment. Data were obtained from C57BL/6 mice following an overnight fast.